#Masslynx software download full
#Masslynx software download windows

Also, background monitoring (e.g., phospholipids) to prevent matrix effects during method development is simplified. This qualitative information can be extracted at a later stage through retrospective analysis of the data.

#Masslynx software download full

In HRMS full scan mode, information on metabolites and/or biomarkers can be simultaneously obtained with the quantitative data. The newest generation high-resolution MS instrumentation offers not only higher resolution and better mass accuracy, but also better sensitivity and a larger dynamic range. These instruments have the potential to become of greater interest in quantitative analysis in different fields of pharmaceutical research, e.g., peptide analyses and quan-qual approaches. HRMS allows both full scan and product ion scan workflows for quantitative analysis. On a triple quad mass spectrometer, quantitation is realized in selected or multiple reaction monitoring mode (SRM/MRM) while in HRMS, the extracted chromatograms are only generated post-acquisition. Nevertheless, high-resolution mass spectrometry (HRMS) has been piloted as alternative platform for quantitative analysis. In the pharmaceutical industry, liquid chromatography (LC) coupled to a triple quad mass spectrometer (MS) is in general the standard approach for quantitative analysis because excellent robustness, selectivity, and sensitivity can be achieved.

Quantitative analysis of small molecules plays a significant role in many research disciplines. Simple guidelines are provided for the selection of an optimal mass extraction window for continuum and centroided data. The impact of these differences on quantitative HRMS performance was evaluated on calibration curves of eight small molecule compounds in plasma using four different ways of processing.

These differences are illustrated on HRMS data acquired for the same plasma samples on three different platforms, i.e., a Sciex QToF, a Waters QToF, and a Thermo Orbitrap system, and processed in four different software packages, i.e., Sciex AnalystВ® TF, Waters Masslynx, Waters Unifi, and Thermo Xcalibur. The selection of centroid versus continuum/profile data for processing, selection of the optimal narrow mass extraction window, using theoretical versus measured accurate mass for the extraction of the ion chromatograms as well as differences in calculations and data handling residing in the different vendor software packages are tackled in the presented manuscript. Optimal processing of HRMS data requires different considerations and software tools and can have an impact on data processing and final results.

#Masslynx software download windows

Quantitative processing of HRMS data is performed based on narrow mass extraction windows rather than on nominal mass product ion chromatograms (SRM or MRM). This, in combination with the improved performance of the new generation HRMS systems, triggers more (bio) analysts to switch from triple quad MS systems to HRMS for quantitative analysis.

High-resolution mass spectrometry (HRMS) in full scan mode acquires all ions present in the sample of interest offering a lot of qualitative information.